Nucleoli Marker Antibody [NCM102-1]
| Code | Size | Price |
|---|
| PSI-33-890-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: NCM102-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed): 0.5-1.0 ug/ml for 30 min at RT
IHC (Formalin-paraffin): 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Nucleoli marker antibody may be required for optimal performance.
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed): 0.5-1.0 ug/ml for 30 min at RT
IHC (Formalin-paraffin): 0.5-1.0 ug/ml for 30 min at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the Nucleoli marker antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
The nucleolus is the largest structure in the nucleus of eukaryotic cells, where it primarily serves as the site of ribosome synthesis and assembly. Nucleoli also have other important functions like assembly of signal recognition particles and playing a role in the cell's response to stress. Nucleoli are made of proteins and RNA and form around specific chromosomal regions. [Wiki]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A preparation of myeloid leukemia cell nuclei was used as the immunogen.
NCBI Gene ID #:
Unknown
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher