PAX7 Antibody [PAX7/497]

ProSci
Product Code: PSI-33-184
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-184-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: PAX7/497
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of human HeLa cell lysate with PAX7 antibody (clone PAX7/497). Expected molecular weight: 55-57 kDa.

Western blot testing of human HeLa cell lysate with PAX7 antibody (clone PAX7/497). Expected molecular weight: 55-57 kDa.

Further Information

Additional Names:
Paired box protein Pax-7, HuP1, PAX7, HUP1
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Background:
The PAX gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the PAX family of proteins have been linked to disease and cancer in humans. PAX7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that PAX7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding PAX7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
NCBI Gene ID #:
5081
NCBI Official Name:
paired box 7
NCBI Official Symbol:
PAX7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P23759
User NOte:
Optimal dilutions for each application to be determined by the researcher