SUMO2/3 Antibody [S23MT-1]
| Code | Size | Price |
|---|
| PSI-33-933-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: S23MT-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
IF: 0.5-1 ug/ml
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the SUMO2/3 antibody may be required for optimal performance.
WB: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
Titering of the SUMO2/3 antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. SUMO proteins are synthesized as precursors that undergo processing before conjugation to target proteins. Sumoylation targets for a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. SUMO2/3 contribute to a greater percentage of protein modification than does SUMO1 and they can form polymeric chains. In addition, SUMO3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer?s disease.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human SUMO2 protein was used as the immunogen for this SUMO2/3 antibody. This antibody reacts with both SUMO2 and SUMO3.
NCBI Gene ID #:
6613, 6612
NCBI Official Name:
small ubiquitin-like modifier 2, small ubiquitin-like modifier 3
NCBI Official Symbol:
SUMO2, SUMO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P61956, P55854
User NOte:
Optimal dilutions for each application to be determined by the researcher