Helicobacter Pylori Antibody

ProSci
Product Code: PSI-33-248
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-248-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Formalin/paraffin human stomach stained with Helicobacter pylori antibody.

Formalin/paraffin human stomach stained with Helicobacter pylori antibody.

Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Background:
The spiral shaped bacterium Helicobacter pylori is strongly associated with inflammation of the stomach and is also implicated in the development of gastric malignancy. This bacteria is known to cause peptic ulcers and chronic gastritis in human. It is associated with duodenal ulcers and may be involved in development of adenocarcinoma and low-grade lymphoma of mucosa associated lymphoid tissue in the stomach. This antibody stains the individual Helicobacter pylori bacterium when it presents on the surface of the epithelium or in the cytoplasm of the epithelial cells in biopsy tissue sections from the antrum and body of the stomach.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Total sonicate of Helicobacter ylori was used as the immunogen for this antibody.
NCBI Gene ID #:
Not Applicable
Physical State:
Liquid
Purification:
Protein A affinity chromatography
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher