Connexin 32 Antibody [R5.21C]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (Frozen): 1-2 ug/ml for 30 min at RT

Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

14618

gap junction protein, beta 1

Gjb1

Mus musculus

Liquid

Protein G affinity chromatography

Neuroscience

P28230

Optimal dilutions for each application to be determined by the researcher