p53 Antibody [PAb 122]

Product Code: PSI-33-854

Supplier: ProSci

Code	Size	Price

PSI-33-854-100ug

100ug

£765.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2b, kappa

Antibody Clonality: Monoclonal

Antibody Clone: PAb 122

Regulatory Status: RUO

Target Species:

Hamster
Human
Monkey
Mouse
Rat

Applications:

Immunofluorescence (IF)
Immunohistochemistry- Frozen Section (IHC-F)
Western Blot (WB)

Shipping:

blue ice

Storage:

Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using p53 antibody (clone PAb 122). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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Documents

Further Information

Additional Names:

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Application Note:

Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 0.5-1 ug/ml

IHC (Frozen) only: 0.5-1 ug/ml for 30 min at RT

Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.

Background:

This antibody is specific to a conserved determinant of the p53 protein. PAb 122 binds to the C-terminus (aa 370-378) of both wild type and mutated p53. When microinjected into nuclei, PAb 122 blocked re-entry into the S-phase of the cell cycle. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. p53 binds to a DNA consensus sequence, the p53 response element, and it regulates normal cell growth cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). The protein in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Its the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation.

Buffer:

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration:

0.2 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminal end (aa 370-378) of p53.

NCBI Gene ID #:

7157

NCBI Official Name:

tumor protein p53

NCBI Official Symbol:

TP53

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Purification:

Protein G affinity chromatography

Research Area:

Cancer,Cell Cycle,Signal Transduction,

Swissprot #:

P04637

User NOte:

Optimal dilutions for each application to be determined by the researcher