Kappa Light Chain Antibody [KLC709]

Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells

IHC (Frozen

FFPE): 0.5-1.0 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-Kappa light chain antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This antibody is specific to the kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general, the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Recombinant human Kappa light chain was used as the immunogen for this anti-Kappa light chain antibody.

3514

immunoglobulin kappa constant

IGKC

Homo sapiens

Liquid

Protein G affinity chromatography

Immunology

P01834, P01601

Optimal dilutions for each application to be determined by the researcher