HCG-alpha Antibody [HCGa/53]

ProSci
Product Code: PSI-33-112
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-112-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HCGa/53
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

1 / 1
IHC testing of human placenta stained with HCG-alpha antibody (HCGa/53). Note specific membrane staining.

IHC testing of human placenta stained with HCG-alpha antibody (HCGa/53). Note specific membrane staining.

Further Information

Additional Names:
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, CG-alpha, Follicle-stimulating hormone alpha chain, FSH-alpha, Follitropin alpha chain, Luteinizing hormone alpha chain, LSH-alpha, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, TSH-alpha, Thyrotropin alpha chain, CGA
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Variations in protocols, secondaries and substrates may require the HCG-alpha antibody to be titered for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
This antibody reacts with a protein of ~13kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. HCG is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein was used as the immunogen for this HCG-alpha antibody.
NCBI Gene ID #:
1081
NCBI Official Name:
glycoprotein hormones, alpha polypeptide
NCBI Official Symbol:
CG alpha
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified monoclonal antibody
Research Area:
Cancer,Cell Cycle,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P01215
User NOte:
Optimal dilutions for each application to be determined by the researcher