EGF Receptor Antibody [B1D8]
| Code | Size | Price |
|---|
| PSI-33-120-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: B1D8
Regulatory Status: RUO
Target Species: Human
Application: Immunoprecipitation (IP)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Application Note:
Functional Activity (Inhibition): antibody blocks EGF receptor activation
inhibits A431 cell proliferation
arrests tumor growth in vivo
affinity purification
IP: 1-2?g/500ug protein lysate
Flow Cytometry: 0.5-1 ug/million cells
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
inhibits A431 cell proliferation
arrests tumor growth in vivo
affinity purification
IP: 1-2?g/500ug protein lysate
Flow Cytometry: 0.5-1 ug/million cells
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Background:
This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa?induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.?EGFR?is type I receptor tyrosine kinase with sequence homology to?ErbB2, -3 -4 or?HER2, -3 -4. It binds to Epidermal Growth Factor (EGF),?Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Microsomes from A431 cells were used as the immunogen for this antibody.
NCBI Gene ID #:
1956
NCBI Official Name:
epidermal growth factor receptor
NCBI Official Symbol:
EGF R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified monoclonal antibody
Research Area:
Cancer,Signal Transduction
SPECIFICITY:
Does not react with mouse and rat
Swissprot #:
P00533
User NOte:
Optimal dilutions for each application to be determined by the researcher