Blood Group Lewis y Antibody [A70-A/A9]

ProSci
Product Code: PSI-33-676
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-676-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: A70-A/A9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human colon carcinoma with Lewis y antibody (clone A70-A/A9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Further Information

Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the Lewis y antibody should be determined by the researcher.
Background:
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
Not Applicable
User NOte:
Optimal dilutions for each application to be determined by the researcher