Ku70 + Ku80 Antibody [KU729]
| Code | Size | Price |
|---|
| PSI-33-138-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: KU729
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunocytochemistry (ICC)
- Immunofluorescence (IF)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
X-ray repair cross-complementing protein 6, 364-, 4299-, 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Ku70, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 6, XRCC6, G22P1
Application Note:
Flow cytometry: 0.5-1ug/10e6 cells
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 ug/ml for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
IF: 0.5-1.0 ug/ml
Immunocytochemistry (Acetone-fixed cells): 0.25-0.5 ug/ml for 30 minutes at RT
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Background:
This antibody recognizes a dimer of two proteins of 70kDa (Ku70) and ~80kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognizes a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
NCBI Gene ID #:
2547
NCBI Official Name:
X-ray repair complementing defective repair in Chinese hamster cells 6
NCBI Official Symbol:
XRCC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified monoclonal antibody
Research Area:
Cancer
SPECIFICITY:
Does not react with mouse, rat, chicken
Swissprot #:
Q6FG89
User NOte:
Optimal dilutions for each application to be determined by the researcher