CD45RA Antibody [158-4D3]

Product Code: PSI-33-195

Supplier: ProSci

Code	Size	Price

PSI-33-195-100ug

100ug

£765.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Mouse

Antibody Isotype: IgG2a, kappa

Antibody Clonality: Monoclonal

Antibody Clone: 158-4D3

Regulatory Status: RUO

Target Species: Human

Applications:

Immunofluorescence (IF)
Immunohistochemistry (IHC)

Shipping:

blue ice

Storage:

Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC staining of FFPE human tonsil with CD45RA antibody (clone 158-4D3). HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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Flow cytometry testing of human Jurkat cells with CD45RA antibody (clone 158-4D3); Red=isotype control, Blue= CD45RA antibody.

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Western blot testing of human spleen lysate with CD45RA antibody (clone 158-4D3). Expected molecular weight: 147-220 kDa depending on glycosylation level.

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SDS-PAGE analysis of purified, BSA-free CD45RA antibody (clone 158-4D3) as confirmation of integrity and purity.

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Documents

Further Information

Additional Names:

Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45

Application Note:

Flow Cytometry: 0.5-1 ug/million cells

IF: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RA antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:

This antibody recognizes a protein of 205kDa-220kDa, identified as CD45RA, an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ Tcells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin Tcells. T-cells expressing CD45RO are memory Tcells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral Tcells. This CD45RA antibody is useful in the study of the subpopulation of CD4+ or CD8+ T-cells. It is especially useful in differentiating T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Buffer:

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration:

0.2 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Stimulated human leukocytes were used as the immunogen for this CD45RA antibody.

NCBI Gene ID #:

5788

NCBI Official Name:

protein tyrosine phosphatase, receptor type, C

NCBI Official Symbol:

PTPRC

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Purification:

Protein G affinity chromatography

Research Area:

Cancer,Immunology,Neuroscience,Signal Transduction

Swissprot #:

P08575

User NOte:

Optimal dilutions for each application to be determined by the researcher