Cyclin B1 Antibody [V92.1]
| Code | Size | Price |
|---|
| PSI-33-075-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: V92.1
Regulatory Status: RUO
Target Species:
- Hamster
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunoprecipitation (IP)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
G2/mitotic-specific cyclin-B1, CCNB1, CCNB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IP: 1-2ug/500ug protein--precipitates active Cyclin B1 complexes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
IF: 1-2 ug/ml
IP: 1-2ug/500ug protein--precipitates active Cyclin B1 complexes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Background:
This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Hamster protein was used as the immunogen for this Cyclin B1 antibody.
NCBI Gene ID #:
891
NCBI Official Name:
cyclin B1
NCBI Official Symbol:
CCNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Cell Cycle
Swissprot #:
P14635
User NOte:
Optimal dilutions for each application to be determined by the researcher