Cytokeratin 10 Antibody [LH2]
| Code | Size | Price |
|---|
| PSI-33-167-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: LH2
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8-9, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
This antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). cytokeratin 10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of cytokeratin 10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, cytokeratin 10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), cytokeratin 10 is detected very frequently. Expression of cytokeratin 10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Skin extract from a human psoriasis patient was used as the immunogen for this Cytokeratin 10 antibody.
NCBI Gene ID #:
3858
NCBI Official Name:
keratin 10
NCBI Official Symbol:
KRT10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P13645
User NOte:
Optimal dilutions for each application to be determined by the researcher