Muscle Actin Antibody [HHF35]
| Code | Size | Price |
|---|
| PSI-33-235-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: HHF35
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rabbit
- Rat
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1, ACTA
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Background:
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
NCBI Gene ID #:
58
NCBI Official Name:
actin, alpha 1, skeletal muscle
NCBI Official Symbol:
ACTA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Signal Transduction
Swissprot #:
P68133
User NOte:
Optimal dilutions for each application to be determined by the researcher