TAG-72 Antibody [TAGP-4]
| Code | Size | Price |
|---|
| PSI-33-907-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TAGP-4
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.
1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Background:
This antibody recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 antibody with a GCDFP-15 antibody is valuable in the diagnosis of apocrine carcinoma.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human TAG-72 protein was used as the immunogen for this antibody.
NCBI Gene ID #:
182875
NCBI Official Name:
mRNA cap guanine-N7 methyltransferase
NCBI Official Symbol:
tag-72
NCBI Organism:
Caenorhabditis elegans
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q9XVS1
User NOte:
Optimal dilutions for each application to be determined by the researcher