Biotin Antibody [Hyb-8]
| Code | Size | Price |
|---|
| PSI-33-236-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: Hyb-8
Regulatory Status: RUO
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
WB: 2-5 ug/ml
Confocal Microscopy
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
WB: 2-5 ug/ml
Confocal Microscopy
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.
1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Background:
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher