Multi Cytokeratin 4/5/6/8/10/13/18 Antibody [C11]

Flow Cytometry: use the Multi Cytokeratin mAb at 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Multi Cytokeratin antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This antibody recognizes multi cytokeratins: 4, 5, 6, 8, 10, 13, and 18. It is a broad-spectrum cytokeratin antibody which has been reported to be used to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of cytokeratin markers in cancer research and tumor diagnosis.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

A keratin-enriched preparation from cultured human A431 was used as the immunogen for this Multi Cytokeratin antibody.

3851 (CK4); 3852 (CK5); 3853 (CK6); 3856 (CK8); 3858 (CK10); 3860 (CK13); 3875 (CK18)

Liquid

Protein G affinity chromatography

Signal Transduction

Optimal dilutions for each application to be determined by the researcher