EMA Antibody [MUC1/520]

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ProSci
Product Code: PSI-33-266
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-266-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: MUC1/845
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).
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Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).
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Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
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Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
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SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MUC1 / EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
Formalin-fixed, paraffin-embedded human breast carcinoma stained with EMA antibody (MUC1/845).
SDS-PAGE Analysis of Purified, BSA-Free EMA Antibody (clone MUC1/845). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EMA antibody to be titered up or down for optimal performance.

1. No pretreatment needed for FFPE staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
This antibody recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of MUC1 (Mucin-1) or EMA (epithelial membrane antigen). The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 / Mucin-1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling through phosphorylations and protein-protein interactions. In immunohistochemical assays, the MUC1 / EMA antibody superbly stains routine formalin/paraffin carcinoma tissues. MUC1 antibody is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human milk fat globule membranes were used as the immunogen for this EMA antibody.
NCBI Gene ID #:
4582
NCBI Official Name:
mucin 1, cell surface associated
NCBI Official Symbol:
MUC1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P15941
User NOte:
Optimal dilutions for each application to be determined by the researcher