CD3e Antibody

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ProSci
Product Code: PSI-33-082
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-082-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Cavia (Guinea pig)
  • Chicken (Gallus)
  • Feline (cat)
  • Frog
  • Hamster
  • Horse
  • Human
  • Mouse
  • Opossum
  • Porcine (pig)
  • Rabbit
  • Rat
  • Sheep
Applications:
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.
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Western blot testing of human Jurkat lysate in the absence and presence of immunizing peptide, and mouse thymus lysate probed with CD3e antibody.

Documents

Further Information

Additional Names:
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
Application Note:
Flow Cytometry: use the CD3e antibody at 0.5-1 ug/million cells

WB: 0.5-1.0 ug/ml

IP: 1-2ug/500ug protein lysate



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.
Background:
This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
NCBI Gene ID #:
916
NCBI Official Name:
CD3e molecule, epsilon (CD3-TCR complex)
NCBI Official Symbol:
CD3E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A purified CD3e antibody
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P07766
User NOte:
Optimal dilutions for each application to be determined by the researcher