UchL1 Antibody [13C4]

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ProSci
Product Code: PSI-33-222
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-222-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 13C4
Regulatory Status: RUO
Target Species:
  • Bovine (Cattle)
  • Cavia (Guinea pig)
  • Human
  • Mouse
  • Porcine (pig)
  • Rabbit
  • Rat
  • Sheep
  • Zebrafish
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Western blot testing of human brain lysate with UchL1 antibody (clone 13C4). Predicted molecular weight ~25kDa.
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Western blot testing of human brain lysate with UchL1 antibody (clone 13C4). Predicted molecular weight ~25kDa.

Documents

Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, 6---, Neuron cytoplasmic protein 95, PGP 95, PGP95, Ubiquitin thioesterase L1, UCHL1
Application Note:
WB: 0.5-1 ug/ml

IF: 1-2 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the UchL1 antibody to be titered up or down for optimal performance.
Background:
This antibody specifically reacts with a protein of 20-30kDa, identified as UchL1 (Ubiquitin carboxyl-terminal hydrolase-1), or PGP9.5. Studies using UchL1 antibody have shown that expression is related to tumor progression, with high expression correlating with poor prognosis, making the protein a potentially useful marker for pancreatic and invasive colorectal cancer. A mutation in gene is believed to cause a form of Parkinson's disease.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Native protein from brain was used as the immunogen for this UchL1 antibody.
NCBI Gene ID #:
7345
NCBI Official Name:
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
NCBI Official Symbol:
UCHL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P09936
User NOte:
Optimal dilutions for each application to be determined by the researcher