HLA-DP + HLA-DQ + HLA-DR Antibody [CR3/43]

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ProSci
Product Code: PSI-33-732
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-732-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CR3/43
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.
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IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).
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Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red=isotype control, Blue= HLA-DP/DQ/DR antibody.
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IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.
IF/ICC staining of human Raji cells with HLA-DP/DQ/DR antibody (green) and Reddot nuclear stain (red).
Flow cytometry testing of human Raji cells with HLA-DP/DQ/DR antibody at 1ug/10^6 cells (blocked with goat sera); Red=isotype control, Blue= HLA-DP/DQ/DR antibody.

Documents

Further Information

Application Note:
WB: 1-2 ug/ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RT

Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.
Background:
This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa). They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.
NCBI Gene ID #:
3115, 3117, 3119
NCBI Official Name:
major histocompatibility complex, class II, DP beta 1; major histocompatibility complex, class II, DQ alpha 1
NCBI Official Symbol:
HLA-DPB1, HLA-DQA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P04440, P01908, P01909, P01920, P01903
User NOte:
Optimal dilutions for each application to be determined by the researcher