HLA-DRB1 Antibody [L243]
Code | Size | Price |
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PSI-33-394-100ug | 100ug | £765.00 |
Quantity:
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Overview
Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: L243
Regulatory Status: RUO
Target Species:
- Baboon
- Chimpanzee
- Human
- Monkey
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Further Information
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 0.5-1 ug/ml
Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.
Immunofluorescence: 0.5-1 ug/ml
Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.
Background:
This mAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the alpha/beta heterodimer. This mAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
NCBI Gene ID #:
3123
NCBI Official Name:
major histocompatibility complex, class II, DR beta 1
NCBI Official Symbol:
HLA-DRB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P01911
User NOte:
Optimal dilutions for each application to be determined by the researcher