TOP2A Antibody [TPM2A-1]
| Code | Size | Price |
|---|
| PSI-34-064-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TPM2A-1
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells
IF: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
IF: 1-2 ug/ml
Western blot: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Background:
This mAb recognizes a 170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human partial recombinant protein was used as the immunogen for this TOP2A antibody.
NCBI Gene ID #:
7153
NCBI Official Name:
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol:
TOP2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P11388
User NOte:
Optimal dilutions for each application to be determined by the researcher