CELA3B Antibody [CELA3B/1757]
| Code | Size | Price |
|---|
| PSI-33-794-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: CELA3B/1757
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B
Application Note:
Western blot: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
ELISA: 2-4 ug/ml; order BSA free formulation for coating
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the CELA3B antibody should be determined by the researcher.
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 1-2 ug/ml
ELISA: 2-4 ug/ml; order BSA free formulation for coating
IHC (FFPE): 1-2 ug/ml for 30 min at RT
Optimal dilution of the CELA3B antibody should be determined by the researcher.
Background:
This mAb recognizes a protein of ~30kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
NCBI Gene ID #:
23436
NCBI Official Name:
Chymotrypsin-like elastase family member 3B
NCBI Official Symbol:
CELA3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P08861
User NOte:
Optimal dilutions for each application to be determined by the researcher