Cytokeratin 10 Antibody [SPM623]

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ProSci
Product Code: PSI-33-677
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-677-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: SPM623
Regulatory Status: RUO
Target Species:
  • Feline (cat)
  • Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human bladder carcinoma with Cytokeratin 10 antibody (clone SPM623). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human bladder carcinoma with Cytokeratin 10 antibody (clone SPM623). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Documents

Further Information

Additional Names:
Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT

Optimal dilution of the Cytokeratin 10 antibody should be determined by the researcher.
Background:
This mAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 antibody.
NCBI Gene ID #:
3858
NCBI Official Name:
keratin 10
NCBI Official Symbol:
KRT10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P13645
User NOte:
Optimal dilutions for each application to be determined by the researcher