Neuron Specific Enolase Antibody [ENO2/1462]

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ProSci
Product Code: PSI-33-842
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-33-842-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b
Antibody Clonality: Monoclonal
Antibody Clone: ENO2/1462
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Neuron Specific Enolase antibody (clone ENO2/1462). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Neuron Specific Enolase Antibody (clone ENO2/1462). Confirmation of Integrity and Purity of the Antibody.
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Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Neuron Specific Enolase antibody (clone ENO2/1462). Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1462). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free Neuron Specific Enolase Antibody (clone ENO2/1462). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Neuron Specific Enolase antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
This mAb recognizes a protein of about 50 kDa which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 416-433 of human NSE were used as the immunogen for this Neuron Specific Enolase antibody.
NCBI Gene ID #:
2026
NCBI Official Name:
enolase 2 (gamma, neuronal)
NCBI Official Symbol:
ENO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer, Obesity, Neuroscience, Stem Cell
Swissprot #:
P09104
User NOte:
Optimal dilutions for each application to be determined by the researcher