TOP2A Antibody [TOP2A/1362]

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ProSci
Product Code: PSI-33-788
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-788-100ug100ug£765.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG2b, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TOP2A/1362
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human tonsil with TOP2A antibody (clone TOP2A/1362). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

Western blot: 1-2 ug/ml

IHC (FFPE): 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.
Background:
This mAb recognizes an ~170kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 1352-1493 from the human protein were used as the immunogen for this TOP2A antibody.
NCBI Gene ID #:
7153
NCBI Official Name:
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol:
TOP2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P11388
User NOte:
Optimal dilutions for each application to be determined by the researcher