Nuclear Antigen Antibody [NUCM1-1]

Immunofluorescence: 0.5-1 ug/ml

Immunocytochemistry (Acetone-fixed): 0.25-0.5 ug/ml for 30 min at RT

Immunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Nuclear Antigen antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.

Liquid

Protein G affinity chromatography

Other

Not Known

Optimal dilutions for each application to be determined by the researcher