Multi-Cytokeratin Antibody [SPM583]

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Western blot: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

0.2 mg/mL

Unconjugated

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.

3851, 3852, 3853, 3854, 3856, 3858, 3860, 3875

keratin 4, keratin 5, keratin 6A, keratin 6B, keratin 8, keratin 10, keratin 13, keratin 18

KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18

Homo sapiens

Liquid

Protein G affinity chromatography

Signal Transduction

P19013, P13647, P02538, P04259, P48668, P05787, P13645, P13646, P05783

Optimal dilutions for each application to be determined by the researcher