TSHR alpha chain Antibody [SPM223]

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ProSci
Product Code: PSI-33-790
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-790-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: SPM223
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone SPM223). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of FFPE human thyroid carcinoma with TSHR antibody (clone SPM223). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, or 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Documents

Further Information

Additional Names:
TSHR, LGR3, MGC75129, hTSHR-I, CHNG1
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSHR antibody to be titered up or down for optimal performance.
Background:
Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human TSH Receptor alpha chain protein was used as the immunogen for this TSHR antibody.
NCBI Gene ID #:
7253
NCBI Official Name:
thyroid stimulating hormone receptor
NCBI Official Symbol:
TSHR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Membrane, Cancer, Signal Transduction, Immunology
Swissprot #:
P16473
User NOte:
Optimal dilutions for each application to be determined by the researcher