TIGIT Antibody [10B1] (biotin)

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ProSci
Product Code: PSI-RF16058-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-RF16058-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-RF16058-biotin-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 10B1
Regulatory Status: RUO
Target Species: Human
Shipping:
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Storage:
TIGIT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
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A sandwich ELISA was performed using the anti-TIGIT mAb RF16055 (5 μg/ml) as the capture antibody. Biotin-labeled anti-TIGIT mAb RF16058-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.

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Further Information

Additional Names:
TIGIT Antibody: T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Application Note:
TIGIT antibody can be used for as detection antibody in Sandwich Elisa at 1 μg/mL.
Background:
TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH). TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1). Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2).
Background References:
  • Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.
  • Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8(+) T cell effector function. Cancer Cell 2014; 26:923-37.
Buffer:
TIGIT Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIGIT antibody was raised against the extracellular domain of human TIGIT
NCBI Gene ID #:
201633
NCBI Official Name:
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol:
TIGIT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_776160
Protein GI Number:
256600228
Purification:
TIGIT Antibody is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
Q495A1
User NOte:
Optimal dilutions for each application to be determined by the researcher.