TRIM29 Antibody [TPMP29-1]

ProSci
Product Code: PSI-33-930
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-930-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a, kappa
Antibody Clonality: Monoclonal
Antibody Clone: TPMP29-1
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human esophageal carcinoma and TRIM29 antibody (clone TPMP29-1).
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IHC testing of FFPE human esophageal carcinoma and TRIM29 antibody (clone TPMP29-1).

Further Information

Additional Names:
Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein, TRIM29, ATDC
Application Note:
Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the TRIM29 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Tripartite motif-containing protein 29 interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
An N-terminal human recombinant protein fragment was used as the immunogen for the TRIM29 antibody.
NCBI Gene ID #:
23650
NCBI Official Name:
tripartite motif containing 29
NCBI Official Symbol:
TRIM29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cell Cycle
Swissprot #:
Q14134
User NOte:
Optimal dilutions for each application to be determined by the researcher