Villin Antibody [VIL1/1325]

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ProSci
Product Code: PSI-33-727
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-33-727-100ug100ug£765.00
Quantity:
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Overview

Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: VIL1/1325
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.

Images

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IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
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SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.

Documents

Further Information

Additional Names:
VIL1, D2S1471, Villin, Villin 1, Villin-1, VIL
Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 1-2 ug/ml

Western blot: 1-2 ug/ml

IHC (FFPE): 0.25-0.5 ug/ml for 30 min at RT

The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
NCBI Gene ID #:
7429
NCBI Official Name:
villin 1
NCBI Official Symbol:
VIL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Homeostasis
Swissprot #:
P09327
User NOte:
Optimal dilutions for each application to be determined by the researcher