von Willebrand Factor Antibody [3E2D10]
| Code | Size | Price |
|---|
| PSI-33-225-100ug | 100ug | £765.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: 3E2D10
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Shipping:
blue ice
Storage:
Aliquot and Store at -20C. Avoid freez-thaw cycles.
Documents
Further Information
Additional Names:
VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
WB: 0.5-1.0 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the vWF antibody to be titered up or down for optimal performance.
IF: 0.5-1 ug/ml
WB: 0.5-1.0 ug/ml
IHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the vWF antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Background:
von Willebrand Factor is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This vWF antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi?s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 845-949 were used as the immunogen for this vWF antibody.
NCBI Gene ID #:
7450
NCBI Official Name:
von Willebrand factor
NCBI Official Symbol:
VWF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G purified vWF antibody
Research Area:
Cancer, Stem Cell
Swissprot #:
P04275
User NOte:
Optimal dilutions for each application to be determined by the researcher