CD28 Antibody

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ProSci
Product Code: PSI-8683
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8683-0.02mg0.02mg£150.00
Quantity:
PSI-8683-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
blue ice
Storage:
CD28 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of CD28 in human spleen tissue with CD28 antibody at 2 μg/ml.
3 / 3
Immunofluorescence of CD28 in human spleen tissue with CD28 antibody at 20 μg/ml.
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Western blot analysis of CD28 in human spleen tissue lysate with CD28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of CD28 in human spleen tissue with CD28 antibody at 2 μg/ml.
Immunofluorescence of CD28 in human spleen tissue with CD28 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
CD28 Antibody: CD28 Molecule, CD28 Antigen, Tp44, T-Cell-Specific Surface Glycoprotein CD28, T-Cell-Specific Surface Glycoprotein, CD28 Antigen (Tp44)
Application Note:
CD28 antibody can be used for Western blot at 1μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Buffer:
CD28 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (94%), Cow: (94%), Cat: (94%), Rat: (89%), Mouse: (89%)
Immunogen:
CD28 antibody was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human CD28.

The immunogen is located within the last 50 amino acids of CD28.
NCBI Gene ID #:
940
NCBI Official Name:
CD28 molecule
NCBI Official Symbol:
CD28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 28 kDa

Observed: 26 kDa
Protein Accession #:
P10747
Purification:
CD28 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
P10747
User NOte:
Optimal dilutions for each application to be determined by the researcher.