Hydroxystilbamidine bis(methanesulfonate)

2-Hydroxystilbene-4,4'-dicarboxamidine bis(methanesulfonate); Fluoro Gold

Yellow solid.

223769-64-0

32160000

solid

GHS07

Protect from light and moisture.

H315, H319, H335

InChI=1S/C16H16N4O.2CH4O3S/c17-15(18)12-5-2-10(3-6-12)1-4-11-7-8-13(16(19)20)9-14(11)21;2*1-5(2,3)4/h1-9,21H,(H3,17,18)(H3,19,20);2*1H3,(H,2,3,4)/b4-1+;;

YGNSQKCULHSJDC-HFPMQDOPSA-N

Chemical. CAS: 223769-64-0. Formula: C16H16N4O . 2CH4O3S. MW: 280.3 . 192.2. Synthetic. Hydroxystilbamidine (also called Fluoro Gold) is a cationic dye used for staining DNA and RNA. It exhibits distinctively different fluorescence emission profiles when bound to DNA compared to RNA. It is also frequently used as a retrograde neuronal tracer. The fluorescent tracer provides intense retrograde labeling that is extremely sensitive and reliable, does not diffuse out of retrograde-labeled neurons, and can be pressure-injected or introduced by iontophoresis into cells. Hydroxystilbamidine is compatible with most frequently used neuroanatomical techniques such as immunofluorescence, immunocytochemistry, autoradiography, and horseradish peroxidase-based histochemistry, paraffin embedding. Gold color is emitted when tissue is processed with a neutral pH buffer while blue/bright white is emitted when an acidic pH buffer is used. Spectral data: lambdaex=361nm and lambdaem=536nm. In addition, hydroxystilbamidine is an amidine antibiotic with antibacterial antifungal, antitrypanosomal, antimalarial and carcinostatic activities. Binds to DNA and RNA in a non-intercalating manner, and is a powerful inhibitor of ribonucleases. Binds also to lysosomes.

MFCD01863083

C16H16N4O . 2CH4O3S

280.3 . 192.2

Vial

P261, P305+P351+P338

Hydroxystilbamidine (also called Fluoro Gold) is a cationic dye used for staining DNA and RNA. It exhibits distinctively different fluorescence emission profiles when bound to DNA compared to RNA. It is also frequently used as a retrograde neuronal tracer. The fluorescent tracer provides intense retrograde labeling that is extremely sensitive and reliable, does not diffuse out of retrograde-labeled neurons, and can be pressure-injected or introduced by iontophoresis into cells. Hydroxystilbamidine is compatible with most frequently used neuroanatomical techniques such as immunofluorescence, immunocytochemistry, autoradiography, and horseradish peroxidase-based histochemistry, paraffin embedding. Gold color is emitted when tissue is processed with a neutral pH buffer while blue/bright white is emitted when an acidic pH buffer is used. Spectral data: lambdaex=361nm and lambdaem=536nm. In addition, hydroxystilbamidine is an amidine antibiotic with antibacterial antifungal, antitrypanosomal, antimalarial and carcinostatic activities. Binds to DNA and RNA in a non-intercalating manner, and is a powerful inhibitor of ribonucleases. Binds also to lysosomes.

>95% (HPLC)

Warning

OC1=C(/C=C/C2=CC=C(C([NH3+])=N)C=C2)C=CC(C([NH3+])=N)=C1.CS(=O)([O-])=O.CS(=O)([O-])=O

Soluble in water or DMSO (20mg/ml).

Synthetic

Non-hazardous

Fluorescent Reagents

41105331

Stable for at least 2 years after receipt when stored at +4°C.