Sheep anti Mouse Disabled-1 (NT)

Immunoblotting at 1-5 ?g/ml, Immunoprecipitation at 5-10?g/ml. Optimal concentration should be evaluated by serial dilutions.

Disruption of the disabled-1 gene (Dab1) results in aberrant migration of neurons during development and disorganization of laminar structures throughout the brain. Dab1 is thought to function as an adaptor molecule in signal transduction processes. It contains a protein-protein interaction (PI) similar to the phosphotyrosine binding domain of the Shc oncoprotein, it is phosphorylated by the Src protein tyrosine kinase, and it binds to SH2 binding domains in a phosphotyrosine dependent manner. Disabled ?1 acts downstream of reelin in a signalling pathway.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as 0.2mm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Western Blotting

Recombinant protein corresponding to amino acids 1-179 of mouse disabled coupled to a GST fusion protein.

293 cells transfected with Dab-1 HA tag

Unconjugated

Products are stable for one year from purchase when stored properly

Ammonium Sulfate Precipitation

Goats were immunized with a recombinant protein corresponding to amino acids 1-179 of mouse disabled coupled to a GST fusion protein.

Disabled homolog 1; DAB1

P97318 (Mouse)