Rabbit anti Human Phosphatidic Acid Phosphatase 2B
| Code | Size | Price |
|---|
| EXA-X1651P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme Immunoassay (EIA)
- Western Blot (WB)
Shipping:
Ship on dry ice freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Documents
Further Information
Applications Description:
Antibody can be used for Western blotting (see vial for dilution) and EIA. Optimal concentration should be evaluated by serial dilutions.
Background:
Vascular endothelial growth factor and type I collagen inducible protein (VCIP), also known as phosphatidic acid phosphatase 2b (PAP2b), was originally identified in a functional assay of angiogenesis. VCIP/PAP2b exhibits an Arg?Gly?Asp (RGD) cell adhesion sequence. Immunoprecipitation and fluorescence-activated cell sorting analyses demonstrated that VCIP-RGD is exposed to the outside of the cell surface. Retroviral transduction of VCIP can induce cell aggregation and cell? cell interactions, modestly increase p120 catenin expression and promote activation of the Fak, Akt and GSK3? protein kinases. Expression of VCIP promoted adhesion, spreading and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b/VCIP is expressed in close proximity to vascular endothelial growth factor, von Willebrand factor and {alpha}v?3 integrin in tumor vasculatures.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Lot specific, see vial
Field of Interest:
Signal Transduction
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the phosphatidic acid phosphatase 2B protein
Positive Control:
Human brain lysate (Cat. No. X1633C)
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Antigen Immunoaffiinity Purification
Synonyms:
PAP2B
UniProt:
O14495
References
[1] Kai M., Wada I., Imai S.-I., Sakane F., Kanoh H.; Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase.; J. Biol. Chem. 272:24572-24578(1997).
[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).
[3] Humtsoe J.O., Feng S., Thakker G.D., Yang J., Hong J., Wary K.K.; Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.; EMBO J. 22:1539-1554(2003).
[4] Leung D.W., Tompkins C.K.; Molecular cloning of and expression of an isoform of human phosphatidic acid phosphatase cDNA.; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
[5] Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.; Large-scale concatenation cDNA sequencing.;
Genome Res. 7:353-358(1997).
[6] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[7] Burnett C., Makridou P., Hewlett L., Howard K.; Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity.; BMC Biochem. 5:2-2(2004).
[2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998).
[3] Humtsoe J.O., Feng S., Thakker G.D., Yang J., Hong J., Wary K.K.; Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.; EMBO J. 22:1539-1554(2003).
[4] Leung D.W., Tompkins C.K.; Molecular cloning of and expression of an isoform of human phosphatidic acid phosphatase cDNA.; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
[5] Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.; Large-scale concatenation cDNA sequencing.;
Genome Res. 7:353-358(1997).
[6] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
[7] Burnett C., Makridou P., Hewlett L., Howard K.; Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity.; BMC Biochem. 5:2-2(2004).