Mouse anti Phosphotyrosine conjugated with peroxidase

Working dilution of 1:2,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years, While the phosphorylation of specific tyrosine residues has be shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved invaluable to the study of tyrosine-phosphorylated proteins and the biochemical pathways in which they fuction. The HRP conjugate of PY20 anti-phosphotyrosine is especailly useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immuncytochemical protcols in situations wherin the use of a secondary antibody would produce undesirable background signals.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided in 50% glycerol, 0.02M sodium phosphate, pH 7.5, 0.15M sodium chloride

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

HRP

A431 cells stimulated with EGF(Exalpha Cat # X1003)

Provided in 60% glycerol, phosphate buffered saline, and 0.1% 2-Chloroacetamide

HRP conjugate

Products are stable for one year from purchase when stored properly

Prot A/G

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Mouse anti phosphotyrosine, PY20, HRP conjugate, Horseradish peroxidase conjugate

N/A