Mouse anti Phosphotyrosine conjugated with biotin

Application: A dilution of 1:5,000 is suggested for western blot or ELISA, 1:500 for immunohistochemistry or immunocytochemistry.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated proteins biochemical pathways in which they function. The biotin conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of phosphotyrosyl proteins in western blotting, immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would produce unacceptable background signals. In addition, the strength of the biotin-streptavidin interaction and the immense variety of streptavidin-coupled probes available make PY20-biotin a very versatile reagent.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Signal Transduction

Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide

Western Blotting

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Biotin

Biotin

Products are stable for one year from purchase when stored properly

Prot A/G

Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.

Mouse anti phosphotyrosine, PY20, Biotin Conjugate

N/A