Mouse anti HA Tag conjugated with agarose

Use 25?l of slurry/100?g of lysate for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.

A nine amino acid sequence derived from the viral hemagglutinin is frequently incorperated into recombinant proteins as an expression tag. Clone 12CA5 anti-HA is useful for western blotting and immunoprecipitation of these proteins. Shown is a typical western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-family GTPase, expressed in HEK293 cells and immunoprecipitated with anti-HA agarose (lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (lane 2), and control HEK293 cells (lane 3) probed with clone 14G7 anti-Rit. Reacts with HA tagged proteins.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Molecular Biology

Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, and 3 mM sodium azide

Western Blotting

Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin

HA taged proteins

Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, 0.08% sodium azide

Agarose

Reagents are stable for the period shown on the vial label when stored properly

Balb/C mice were immunized with a 9 amino acid petide of influenza virus hemagglutinin

Mouse anti HA, Agarose Conjugate, hemaglutinin

Q3TBD2