Mouse anti Green Fluorescent Protein (GFP)

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Nordic MuBio
Product Code: EXA-X1574M
Product Group: Primary Antibodies
Supplier: Nordic MuBio
CodeSizePrice
EXA-X1574M100 ug£316.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: F56-BA1.2.3
Regulatory Status: RUO
Application: Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Further Information

Applications Description:
Antibody can be used for Western blotting (1-10 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Green Fluorescent Protein (GFP) from Jellyfish (Aequorea Victoria) is a 27kDa protein that provides strong visible fluorescence. GFP can be used as a reporter of gene expression, a tracer of cell lineage, and a fusion tag to monitor protein localization from living cells. It can also be used to access protein-protein interactions in yeast two hybrid systems and measure distances between proteins in fluorescence energy transfer (FRET).
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Molecular Biology
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.
Positive Control:
Purified Green Fluorescent Protein
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Protein A/G Chromatography
Source:
Hybridoma produced by the fusion of splenocytes of mice immunized with full-length green fluorescent protein and mouse myeloma cells.
UniProt:
P42212

References

1. Prasher,D.C et.al. 'Primary structure of the Aequorea victoria green-fluorescentprotein.' Gene 111 (2), 229-233 (1992)

2. Inouye,S. and Tsuji,F.I. 'Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein' FEBS Lett. 341 (2-3), 277-280 (1994)

3. Cody,C.Wet.al. 'Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein' Biochemistry 32 (5), 1212-1218 (1993