Rabbit anti Human Netrin 1
| Code | Size | Price |
|---|
| EXA-X1873P | 100 ug | £316.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Bovine (Cattle)
- Feline (cat)
- Human
- Mouse
- Rat
- Rhesus Macaque
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Further Information
Applications Description:
Antibody can be used for Western blotting (0.5-2 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Background:
Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis. Netrin 1?s main receptor is DCC (deleted in colorectal cancer- a putative tumor suppressor gene whose expression is lost in numerous cancers). DCC belongs to the so-called family of dependence receptors. These receptors induce apoptosis when their ligand is absent, thus conferring a state of cellular dependence on Netrin 1 availability. The DCC/netrin-1 duo regulates neuron survival during nervous system development. The mechanism(s) by which Netrin 1/ DCC triggers cell death are still unknown. The localization of DCC to lipid rafts appears to be a prerequisite for its proapoptotic activity.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Apoptosis
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Synthetic peptide derived from the human Netrin 1 protein
Positive Control:
Antibody tested on MDCKII cell lysates.
Product Form:
Unconjugated
Product Stability:
Products are stable for one year from purchase when stored properly
Purification Method:
Ammonium Sulfate Precipitation
Synonyms:
Netrin 1, NTN1
UniProt:
NP_004813
References
1. Nguyen, A., et al. 'Netrin-1 induces angiogenesis via a DCC-dependent ERK 1/2-eNOS feed-forward mechanism.' Proc. Natl. Acad. Sci. USA, 2006, 103, 6530-6535
2. Shekarabi, M., et al. 'Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling comlex that promotes growth cone expansion.' J. Neurosci. 2005, 25, 3132-3141
3. Jarjour, A.A., et al. 'Netrin-1 is a chemorepellent for oligodendrocyte precursor cells in the embryonic spinal cord.' J. Neurosci. 2003, 23, 3735-3744
4. Furne C, et al. 'The dependence receptor DCC requires lipid raft localization for cell death signaling.' Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4128-33. Epub 2006 Mar 6.??
5. Furne C, Mehlen P. 'The deadly rafts: when lipid rafts regulate dependence receptors.' Med Sci (Paris). 2006 Nov;22(11):909-10.
6. Nakamura F, Takei K, Goshima Y. 'Signaling mechanism connecting axon guidance molecules and growth cone cytoskeleton.' Tanpakushitsu Kakusan Koso. 2006 May;51(6 Suppl):733-41.
7. Chang C, et al. 'MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrin.' Curr Biol. 2006 May 9;16(9):854-62. Epub 2006 Apr 20.
PMID: 16618541
8. Xie Y, et al. 'DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation.' J Biol Chem. 2006 Feb 3;281(5):2605-11. Epub 2005 Nov 30.
2. Shekarabi, M., et al. 'Deleted in colorectal cancer binding netrin-1 mediates cell substrate adhesion and recruits Cdc42, Rac1, Pak1, and N-WASP into an intracellular signaling comlex that promotes growth cone expansion.' J. Neurosci. 2005, 25, 3132-3141
3. Jarjour, A.A., et al. 'Netrin-1 is a chemorepellent for oligodendrocyte precursor cells in the embryonic spinal cord.' J. Neurosci. 2003, 23, 3735-3744
4. Furne C, et al. 'The dependence receptor DCC requires lipid raft localization for cell death signaling.' Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4128-33. Epub 2006 Mar 6.??
5. Furne C, Mehlen P. 'The deadly rafts: when lipid rafts regulate dependence receptors.' Med Sci (Paris). 2006 Nov;22(11):909-10.
6. Nakamura F, Takei K, Goshima Y. 'Signaling mechanism connecting axon guidance molecules and growth cone cytoskeleton.' Tanpakushitsu Kakusan Koso. 2006 May;51(6 Suppl):733-41.
7. Chang C, et al. 'MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrin.' Curr Biol. 2006 May 9;16(9):854-62. Epub 2006 Apr 20.
PMID: 16618541
8. Xie Y, et al. 'DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation.' J Biol Chem. 2006 Feb 3;281(5):2605-11. Epub 2005 Nov 30.