Mouse anti Human Caspase-7

Detects proenzyme form of human Caspase-7 by Western blot. Optimal concentration should be evaluated by serial dilutions.

Caspases are key effectors of programmed cell death. Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 is a 303 amino acid protein with high similarity to caspase-3. Caspase-7 occurs in three varient forms. Granzyme B activates pro-caspase-7 to a form which can cleave poly(ADP-ribose) polymerase (PARP) to its signature fragment of ~85 kDa. Also, in vivo caspase-7 appears to be a better substrate for granzyme B than caspase-3. Pro-caspase-7 has been shown to exist as dimers or high order oligomers. Caspase-7 may be an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic T-lymophocyte-induced cell killing in vivo.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

See vial for concentration

Apoptosis

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Western Blotting

Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.

MCF-7 cell line

Unconjugated

Products are stable for one year from purchase when stored properly

Protein A/G Chromatography

Cysteine-requiring Aspartate Protease-7; Apoptotic protease Mch-3; ICE-like apoptotic protease 3

P55210