Mouse anti Human FAS/APO-1 (CD95) conjugated with PE
| Code | Size | Price |
|---|
| EXA-0954 | 100 Tests | £258.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 3.22
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
Activation of either the 55-kD tumor necrosis factor receptor (TNF-R1) or CD95 (Fas/Apo-1) causes apoptosis of cells and liver failure in mice, and has been associated with human liver disorders. The aim of this study was first to clarify the association between CD95 activation, hepatocyte apoptosis, and fulminant liver failure. Next, we investigated whether TNF-R1 and CD95 operate independently of each other in the induction of hepatocyte apoptosis.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Apoptosis
Formulation:
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Full length CD95 protein
Product Form:
RPE
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
hfas -PE, Anti human fas/Apo1 RPE CD95
UniProt:
P25445 (Human)
References
1) The 55-kD tumor necrosis factor receptor and CD95 independently signal murine hepatocyte apoptosis and subsequent liver failure. Author : Leist M; Gantner F; K ?unstle G; Bohlinger I; Tiegs G; Bluethmann H; Wendel A Source : Mol Med, 2(1):109-24 1996 Jan
2) Regulation of CD95 ligand expression: a key element in immune regulation? Author : Brunner T; Yoo NJ; Griffith TS; Ferguson TA; Green DR Source : Behring Inst Mitt, (97):161-74 1996 Oct
3)Expression of CD95 antigen and Bcl-2 protein in non-Hodgkin's lymphomas and Hodgkin's disease [published erratum appears in Am J Pathol 1996 Jul;149(1):346] Author : Nguyen PL; Harris NL; Ritz J; Robertson MJ Source : Am J Pathol, 148(3):847-53 1996 Mar
4) Relation of oxidative stress and glutathione synthesis to CD95(Fas/APO-1)-mediated apoptosis of adult T cell leukemia cells. Author : Kohno T; Yamada Y; Hata T; Mori H; Yamamura M; Tomonaga M; Urata Y; Goto S; Kondo T Source : J Immunol, 156(12):4722-8 1996 Jun 15
5) Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand. Author : M ?uller KP; Mariani SM; Matiba B; Kyewski B; Krammer PH Source : Eur J Immunol, 25(10):2996-9 1995 Oct
6) Activation of the CD95 system increases with disease progression in human immunodeficiency virus type 1-infected children and adolescents. Author : B ?ohler T; B ?aumler C; Herr I; Groll A; Kurz M; Debatin KM Source : Pediatr Infect Dis J, 16(8):754-9 1997 Aug
7) Fc gammaRIII-mediated regulation of hematopoiesis in murine bone marrow cells by interleukin-3 and CD95 (Fas/Apo-1). Author : Yoshikawa H; Sakihama T; Nakajima Y; Tasaka K Source : Blood, 90(5):1911-9 1997 Sep 1
8) Human autoreactive and foreign antigen-specific T cells resist apoptosis induced by soluble recombinant CD95 ligand. Author : Zipp F; Martin R; Lichtenfels R; Roth W; Dichgans J; Krammer PH; Weller M Source : J Immunol, 159(5):2108-15 1997 Sep 1
9) The CD95 (APO-1/Fas) system mediates drug-induced apoptosis in neuroblastoma cells. Author : Fulda S; Sieverts H; Friesen C; Herr I; Debatin KM Research Center, Heidelberg. Source : Cancer Res, 57(17):3823-9 1997 Sep 1
10) Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells. Author : Karawajew L; Wuchter C; Ruppert V; Drexler H; Gruss HJ; D ?orken B; Ludwig W. D. Source : Leukemia, 11(8):1245-52 1997 Aug
2) Regulation of CD95 ligand expression: a key element in immune regulation? Author : Brunner T; Yoo NJ; Griffith TS; Ferguson TA; Green DR Source : Behring Inst Mitt, (97):161-74 1996 Oct
3)Expression of CD95 antigen and Bcl-2 protein in non-Hodgkin's lymphomas and Hodgkin's disease [published erratum appears in Am J Pathol 1996 Jul;149(1):346] Author : Nguyen PL; Harris NL; Ritz J; Robertson MJ Source : Am J Pathol, 148(3):847-53 1996 Mar
4) Relation of oxidative stress and glutathione synthesis to CD95(Fas/APO-1)-mediated apoptosis of adult T cell leukemia cells. Author : Kohno T; Yamada Y; Hata T; Mori H; Yamamura M; Tomonaga M; Urata Y; Goto S; Kondo T Source : J Immunol, 156(12):4722-8 1996 Jun 15
5) Clonal deletion of major histocompatibility complex class I-restricted CD4+CD8+ thymocytes in vitro is independent of the CD95 (APO-1/Fas) ligand. Author : M ?uller KP; Mariani SM; Matiba B; Kyewski B; Krammer PH Source : Eur J Immunol, 25(10):2996-9 1995 Oct
6) Activation of the CD95 system increases with disease progression in human immunodeficiency virus type 1-infected children and adolescents. Author : B ?ohler T; B ?aumler C; Herr I; Groll A; Kurz M; Debatin KM Source : Pediatr Infect Dis J, 16(8):754-9 1997 Aug
7) Fc gammaRIII-mediated regulation of hematopoiesis in murine bone marrow cells by interleukin-3 and CD95 (Fas/Apo-1). Author : Yoshikawa H; Sakihama T; Nakajima Y; Tasaka K Source : Blood, 90(5):1911-9 1997 Sep 1
8) Human autoreactive and foreign antigen-specific T cells resist apoptosis induced by soluble recombinant CD95 ligand. Author : Zipp F; Martin R; Lichtenfels R; Roth W; Dichgans J; Krammer PH; Weller M Source : J Immunol, 159(5):2108-15 1997 Sep 1
9) The CD95 (APO-1/Fas) system mediates drug-induced apoptosis in neuroblastoma cells. Author : Fulda S; Sieverts H; Friesen C; Herr I; Debatin KM Research Center, Heidelberg. Source : Cancer Res, 57(17):3823-9 1997 Sep 1
10) Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells. Author : Karawajew L; Wuchter C; Ruppert V; Drexler H; Gruss HJ; D ?orken B; Ludwig W. D. Source : Leukemia, 11(8):1245-52 1997 Aug