Mouse anti Human CD4 FITC - HLADR PE
| Code | Size | Price |
|---|
| EXA-B4DR | 100 Tests | £368.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
CD4 identifies human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. HLA-DR identifies human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Product Form:
Bi-Test (FITC/RPE) Reagent
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD4 FITC - HLADR PE
UniProt:
P01730, P04233
References
1. Thymus Dependent Membrane Antigens in Man: Inhibition of Cell-Mediated Lympholysis by Monoclonal Antibodies to the TH-2 Antigen. Evans, R.L., Wall, D.W., Platsoucas, C.D., Siegal, F.P., Fikrig, S.M., Testa, C.M, and Good, R.A.. Proc. Nat. Acad. Sci. 78,544,1981.
2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E.. J. Immunology 145(1):102-8,1990.
3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K.. J. Leukoc. Biol. 47(2): 11120,1990.
4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J.. Proc. Natl. Acad. Sci. 87(13):5001-5,1990.
5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J.. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus
6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C.. Nat. 349(6309):533-5,1991.
7. Structural requirements for pairing of alpha and beta chains in HLA-DR and HLA-DP molecules. Karp, D.R., Teletski, C.L., Jaraquemada, D., Maloy, W.L., Coligan, J.E., Long, E.O., J. Exp.. Med. 1990 March ;171(3):615-28.
8. Trans-activation of HLA-DR gene by hepatitis B virus X gene product. Hu K.Q., Vierling J.M., Siddiqui A.. Proc. Natl. Acad. Sci. USA 1990 SEPT.; 87(18):7140-4.
9. Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. Verfaillie C., Blakolmer K., McGlave P., J. Exp. Med. 1990 Aug.: 172(2):509-2.
10. Increased circulating HLA-DR+ CD4+ T cells in systemic lupus erythematosus: alterations associated with prednisolone therapy. Raziuddin, S., Nur, M.A., Al-Wabel, A.A., Scand., Immunol. 1990 Feb.:31(2):139-45.
2. Novel Immunoregulatory Functions of Phenotypically Distinct Subpopulations of CD4+ cells in the Human Neonate. Clement, L.T., Vink, P.E., Bradley, G.E.. J. Immunology 145(1):102-8,1990.
3. Antigen Presentation by the CD4 Positive Monocyte Subset. Szabo, G., Miller, C.L., Kodys, K.. J. Leukoc. Biol. 47(2): 11120,1990.
4. Human Immunodeficiency Virus Infection is Efficiently Mediated by a Glycolipid-Anchored form of CD4. Diamond, D.C., Finberg, R., Chaudhuri, S., Sleckman, B.P., Burakoff, S.J.. Proc. Natl. Acad. Sci. 87(13):5001-5,1990.
5. Development Regulation of the Intrathymic T cell Precursor Population. Adkins, B., J.. Immunol. 146(5):1387-93,1991. 6. Induction of CD4 and Susceptibility to HIV-1 Infection in Human CD8+ T Lymphocytes by Human Herpesvirus
6. Lusso, P., De Maria, A., Malnati, M., Lori, F., DeRocco, S.E., Baseler, M., Gallo, R.C.. Nat. 349(6309):533-5,1991.
7. Structural requirements for pairing of alpha and beta chains in HLA-DR and HLA-DP molecules. Karp, D.R., Teletski, C.L., Jaraquemada, D., Maloy, W.L., Coligan, J.E., Long, E.O., J. Exp.. Med. 1990 March ;171(3):615-28.
8. Trans-activation of HLA-DR gene by hepatitis B virus X gene product. Hu K.Q., Vierling J.M., Siddiqui A.. Proc. Natl. Acad. Sci. USA 1990 SEPT.; 87(18):7140-4.
9. Purified primitive human hematopoietic progenitor cells with long-term in vitro repopulating capacity adhere selectively to irradiated bone marrow stroma. Verfaillie C., Blakolmer K., McGlave P., J. Exp. Med. 1990 Aug.: 172(2):509-2.
10. Increased circulating HLA-DR+ CD4+ T cells in systemic lupus erythematosus: alterations associated with prednisolone therapy. Raziuddin, S., Nur, M.A., Al-Wabel, A.A., Scand., Immunol. 1990 Feb.:31(2):139-45.