Mouse anti Human CD8 FITC - CD45RA PE
| Code | Size | Price |
|---|
| EXA-845RA | 100 Tests | £368.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 (F)/IgG1 (PE)
Antibody Clonality: Monoclonal
Antibody Clone: 17D8/BHKG-2
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Ship at ambient temperature do not freeze refrigerate upon arrival
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
Add 10 ?l of MAB / 100 ?l of 106 PBMC in PBS. Mix Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. Add 10 ?lof MAB / 100 ?l of Whole Blood. Mix gently and incubate for 15 minutes at room temperature 20oC. Lyse Whole Blood and analyze or fix with 0.5% v/v paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
CD8 is useful for the Identification of human T cells suppressor/cytotoxic expressing the 32 kDa M.W. surface antigen. While CD45RA recognizes MW. 220-kdalton of the leucocyte antigen (LCA). CD45RA antigen is a member of the CD45 antigen family that also includes CD45, CD45RO, and CD45RB antigens. CD45RA is expressed on approximately 75% of CD8 lymphocytes and 50% of CD4 lymphocytes, all B cells and NK Cells. CD45RA antigen is expressed on naive T lymphocytes, antigen density decreases upon in vitro activation. A decrease antigen density has been demonstrated for CD4+CD45RA+subset with active multiple sclerosis.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient CD8=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
Product Form:
Bi-Test (FITC/RPE) Reagent
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD8 FITC - CD45RA PE
UniProt:
P01732, P08575
References
1. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259.
2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293.
3. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9.
4. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21.
5. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23.
6. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634
7. CD45 isoform expression on human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM
8. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM
9. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS
10. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H
11. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace D
2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293.
3. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9.
4. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21.
5. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23.
6. Leucocyte Typing IV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R.,et al, eds. Oxford University Press; 1989:628-634
7. CD45 isoform expression on human neonatal T cells: expression and turnover of CD45 isoforms on neonatal versus adult T cells after activation. Cell Immunol 1992 Jun;142(1):114-24 Yamada A; Kaneyuki T; Hara A; Rothstein DM; Yokoyama MM
8. Transient accumulation and subsequent rapid loss of messenger RNA encoding high molecular mass CD45 isoforms after T cell activation. J Immunol 1992 Mar 15;148(6):1898-905 Deans JP; Serra HM; Shaw J; Shen YJ; Torres RM; Pilarski LM
9. Patterns of membrane CD45 isoform expression by leukaemic blasts and normal mature myeloid cells. Int J Hematol 1992 Jun;55(3):235-42 Master PS; Richards SJ; Roberts BE; Scott CS
10. CD45 isoform expression during T cell development in the thymus Eur J Immunol 1992 Jul;22(7):1843-50 Fujii Y; Okumura M; Inada K; Nakahara K; Matsuda H
11. Functional subsets of T cells defined by isoforms of CD45 Biochem Soc Trans 1992 Feb;20(1):184-7 Beverley PC; Daser A; Michie CA; Wallace D