Mouse anti Human CD5 FITC - CD20 PE
| Code | Size | Price |
|---|
| EXA-0520 | 100 Tests | £368.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG2a (F)/IgG1 (PE)
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add 10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
Identification of human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells. Identicication of CD20 PE human B cells associated approximately 10% of peripheral blood lymphocytes.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD5=Derived from the hybridization of mouse NS-1/Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human t-acute lymphoblastic leukemia (ALL) cells. CD20=Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with the LB lymphoblastoid cell line.
Product Form:
Bi-Test (FITC/RPE) Reagent
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD5 FITC - CD20 PE
UniProt:
P06127, P11836
References
1. Anti-DNA Antibody Production by CD5+ and CD5- B Cells of Patients with Systemic Lupus Erythematosus. Suzuki N., Sakane T., Engleman EG, J. Clin. Invest. 1990 Ja;85(1):238-47.
2. Characteristics of CD11c+ CD5+ Chronic B cell Leukemias and the Identification of Novel Peripheral Blood B cell Subsets with Chronic Lymphoid Leukemia Immunophenotypes. Wormsley SB, Baird SM, Gadol N., Rai KR, Sobol RE, Blood 1990 July. 76(1):123-30.
3. Stimulation of CD5 Enhances Signal Transduction by the T cell Antigen Receptor. Imboden JB, June CH, McCutcheon MA, Ledbetter JA, J. Clin. Invest. 1990 Ja;85(1):130-4.
4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5- B Lymphocytes. Nawata Y., Stall Am, Herzenberg LA, Eugui Em, Allison AC, Int. Immunol. 1990;2(7):603-14.
5. Evidence for Differential Responsiveness of Human CD5+ and CD5- B cell Subsets to T cell Independent Mitogens. Zupo S., Dono M., Azzoni L., Chiorazzi N., Ferrarini M., Eur. J. Immunol. 1991 Fe. 21(2):351-9.
6. In Vivo and In Vitro Expression of Myeloid Antigens on B-lineage Acute Lymphoblastic Leukemia Cells. Leukemia, 1991, Ja;5(1):19-25. Hara, J.; Kawa-Ha, K.; Yumura-Yagi, K.; Kurahashi, H.; Tawa, A.; Ishihara, S.; Inoue, M.; Murayama, N.; Okada, S.
7. Activation of Dense Human Tonsilar B Cells. Induction of C-Myc Gene Expression via Two Distinct Signal Transduction Pathways. Immunol., 1991, Feb;146(3):846-53. White, M. W.; McConnnell, F.; Shu, G.L.; Morris, D.R.; Clark, E.A.
8. Differential Effects of Low and High Concentrations of Interleukin 6 on Human B Cell. Eur J. Immunol. 1990 No. 20(11) :2389-93. Levy, Y.; Fermand, J.P; Broute, J.C.
9. Immunophenotypes in Classical B-cell Chronic Lymphocytic Leukemia. Correlation with Normal Cellular Counterpart and Clinical Findings. Cancer, 1990, Oct. 1;66(8):1738-42. Baldini, L.; Cro, L.; Cortelezzi, A.; Calori, R.; Nobili, L.; Maiolo, A.T.; Polli, E. E.
2. Characteristics of CD11c+ CD5+ Chronic B cell Leukemias and the Identification of Novel Peripheral Blood B cell Subsets with Chronic Lymphoid Leukemia Immunophenotypes. Wormsley SB, Baird SM, Gadol N., Rai KR, Sobol RE, Blood 1990 July. 76(1):123-30.
3. Stimulation of CD5 Enhances Signal Transduction by the T cell Antigen Receptor. Imboden JB, June CH, McCutcheon MA, Ledbetter JA, J. Clin. Invest. 1990 Ja;85(1):130-4.
4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5- B Lymphocytes. Nawata Y., Stall Am, Herzenberg LA, Eugui Em, Allison AC, Int. Immunol. 1990;2(7):603-14.
5. Evidence for Differential Responsiveness of Human CD5+ and CD5- B cell Subsets to T cell Independent Mitogens. Zupo S., Dono M., Azzoni L., Chiorazzi N., Ferrarini M., Eur. J. Immunol. 1991 Fe. 21(2):351-9.
6. In Vivo and In Vitro Expression of Myeloid Antigens on B-lineage Acute Lymphoblastic Leukemia Cells. Leukemia, 1991, Ja;5(1):19-25. Hara, J.; Kawa-Ha, K.; Yumura-Yagi, K.; Kurahashi, H.; Tawa, A.; Ishihara, S.; Inoue, M.; Murayama, N.; Okada, S.
7. Activation of Dense Human Tonsilar B Cells. Induction of C-Myc Gene Expression via Two Distinct Signal Transduction Pathways. Immunol., 1991, Feb;146(3):846-53. White, M. W.; McConnnell, F.; Shu, G.L.; Morris, D.R.; Clark, E.A.
8. Differential Effects of Low and High Concentrations of Interleukin 6 on Human B Cell. Eur J. Immunol. 1990 No. 20(11) :2389-93. Levy, Y.; Fermand, J.P; Broute, J.C.
9. Immunophenotypes in Classical B-cell Chronic Lymphocytic Leukemia. Correlation with Normal Cellular Counterpart and Clinical Findings. Cancer, 1990, Oct. 1;66(8):1738-42. Baldini, L.; Cro, L.; Cortelezzi, A.; Calori, R.; Nobili, L.; Maiolo, A.T.; Polli, E. E.