Mouse anti Human CD3 FITC - CD28 PE
| Code | Size | Price |
|---|
| EXA-0328S | 50 Tests | £258.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1 (F)/IgG1 (PE)
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species: Human
Application: Flow Cytometry
Shipping:
Room Temperature
Storage:
Product should be stored at 4-8°C. DO NOT FREEZE
Further Information
Applications Description:
PBMC: Add10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature 20?C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Background:
The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is expressed on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. Identification of human T cells expressing the 22-28,000 M. W. surface antigen. Antihuman CD28 binds the 44kDa MW cell surface protein on the surface of most T cells. CD28 acts as the ligand for the B7/BB-1 molecule on the surface of activated B cells. B7/BB-1 co-stimulates T cells through CD28, along with CD2 and CD3. CD28 antigen is a disulfide-linked homodimeric glycoprotein. The CD28 antigen is present on approximately 60%-80% 0flymphocytes (95% of CD4 and 50% of CD8 lymphocytes). CD28 regulates the expression of cytokines by T cells, not only IL-2, but also IL-1 alpha and CSF-1, usually synthesized by accessory cells. CD28 functions as a cell adhesion molecule (CAM) for certain T cell subsets.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
Titered for flow cytometry
Field of Interest:
Flow Cytometry
Formulation:
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
Functional Analysis:
Flow Cytometry Staining
Immunogen:
CD3=Derived from the hybridization of mouse NS-1 myeloma celss with spleen cells from BALB/c mice immunized with human thymocytes. CD28=Derived from the hybridization of mouse Sp2/O-Ag14 myeloma cells with spleen cells of BALB/c mice immunized with the HPB-ALL T-cell line.
Product Form:
Bi-Test (FITC/RPE) Reagent
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Purification Method:
Protein A/G Chromatography
Synonyms:
CD3 FITC - CD28 PE
UniProt:
P07766, P10747
References
1. Knowles RW. Immunochemical analysis of the T cell-specific antigens. In : Reinhert EL, Haynes BF, Nadl LM and Bernstein ID. eds. Leukocyte Typing II, Human T Lymphocytes. New York, NY: Springer-Verlag; 1986:259.
2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293.
3. Signal transduction via CD4,CD8 and CD28 in mature and immature thymocytes. Implications for thymic selection. Turka LA, Linsley PS, Paine R 3d, Schieven Gl, Thompson GB, Ledbetter JA, J. Immunol. 1991 Mar :146(5): 1428-36.
4. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9.
5. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21.
6. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23.
7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983.
8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987.
9. CD28 is an inducible T cell surface antigen that transduces a proliferative signal in CD3+ mature thymocytes. Turka LA; Ledbetter JA; Lee K; June CH; Thompson CB J Immunol 1990 Mar ;144(5):1646-53
10. CD28 ligation in T-cell activation: evidence for two signal transduction pathways. Ledbetter JA; Imboden JB; Schieven GL; Grosmaire LS; Rabinovitch PS; Lindsten T; Thompson CB; June CH Blood 1990 Apr ;75(7):1531-9
11. T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1. Linsley PS; Clark EA; Ledbetter JA Proc Natl Acad Sci U S A 1990 Ju;87(13):5031-5
12 Role of the CD28 receptor in T-cell activation. June CH; Ledbetter JA; Linsley PS; Thompson CB Immunol Today 1990 Ju;11(6):211-6
13. Differences in surface phenotype and mechanism of action between alloantigen-specific CD8+ cytotoxic and suppressor T cell clones. Koide J; Engleman EG J Immunol 1990 Jan ;144(1):32-40
14. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Linsley PS; Brady W; Grosmaire L; Aruffo A; Damle NK; Ledbetter JA J Exp Med 1991 Mar ;173(3):721-30
15. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. Koulova L; Clark EA; Shu G; Dupont B J Exp Med 1991 Mar ;173(3):759-62
2. Kurrle R. Cluster Report:CD3. In:Knapp W, Dorken B, Gilks WR, Reiber EP, Schmidt RE, Stein H, and von dem Borne AEG Kr, eds. Leukocyte Typing IV, White cell Differentiation Antigens. Oxford, England: Oxford Press 1989:293.
3. Signal transduction via CD4,CD8 and CD28 in mature and immature thymocytes. Implications for thymic selection. Turka LA, Linsley PS, Paine R 3d, Schieven Gl, Thompson GB, Ledbetter JA, J. Immunol. 1991 Mar :146(5): 1428-36.
4. T cell receptor/CD3-signaling induces death by apoptosis in human T cell receptor gamma delta + Tcells. Janssen O, Wesselborg S, Heckl-Ostreicher B, Pechhold K, Bender A, Schondelmaier S, Moldenhauer G, Kabelitz D I Immunol. 1991 Jan146(1):35-9.
5. Clonal analysis of human CD4-CD8-CD3- thymocytes highly purified from postnatal thymus Hori T, Spits H J. Immunol. 1991 Apr 146(7):2116-21.
6. Molecular cloning of the CD3 zeta subunit identifies a CD3 zeta-related product in thymus-derived cells Jin YJ, Claton LK, Howard FD, Koyasu S, Sieh M, Steinbrich R, Tarr GE, Reinherz EL. Proc Natl Acad Sci USA 1990 Ma: 87(9):3319-23.
7. Immunofluorescence Measurement in a Flow Cytometer using Low-Power Helium Neon Laser Excitation. Shapiro, H.M, Glazer, A.N., Christenson, L., Williams, J.M., and Strom, T. B. Cytometry 4,276, 1983.
8. Comparison of Helium Neon and Dye lasers for Excitation of Allophycocyanin. Loken, M.R., Kiej, J.F. and Kelly, K.,A. Cytometry 8, 96, 1987.
9. CD28 is an inducible T cell surface antigen that transduces a proliferative signal in CD3+ mature thymocytes. Turka LA; Ledbetter JA; Lee K; June CH; Thompson CB J Immunol 1990 Mar ;144(5):1646-53
10. CD28 ligation in T-cell activation: evidence for two signal transduction pathways. Ledbetter JA; Imboden JB; Schieven GL; Grosmaire LS; Rabinovitch PS; Lindsten T; Thompson CB; June CH Blood 1990 Apr ;75(7):1531-9
11. T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1. Linsley PS; Clark EA; Ledbetter JA Proc Natl Acad Sci U S A 1990 Ju;87(13):5031-5
12 Role of the CD28 receptor in T-cell activation. June CH; Ledbetter JA; Linsley PS; Thompson CB Immunol Today 1990 Ju;11(6):211-6
13. Differences in surface phenotype and mechanism of action between alloantigen-specific CD8+ cytotoxic and suppressor T cell clones. Koide J; Engleman EG J Immunol 1990 Jan ;144(1):32-40
14. Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Linsley PS; Brady W; Grosmaire L; Aruffo A; Damle NK; Ledbetter JA J Exp Med 1991 Mar ;173(3):721-30
15. The CD28 ligand B7/BB1 provides costimulatory signal for alloactivation of CD4+ T cells. Koulova L; Clark EA; Shu G; Dupont B J Exp Med 1991 Mar ;173(3):759-62